Overview
TALON? Superflow? is a cobalt-based IMAC resin that offers a high-specificity alternative to nickel-charged resins for purifying histidine-tagged proteins.
High specificity – designed for histidine-tagged (his-tagged) purification of recombinant proteins when purity is more important than yield.
Different selectivity compared to nickel-charged resins – an alternative to nickel affinity resins when a different metal ion is required.
High-specificity affinity resin for IMAC chromatography
TALON Superflow is used to purify his-tagged proteins from prokaryotic or eukaryotic expression systems. It is suitable for purification under native or denaturing conditions, and it can bind up to 20 mg of his-tagged protein per mL resin. The resin is compatible with commonly used IMAC reagents.
His-tagged proteins sometimes require cobalt-charged IMAC resin when nickel is not the optimal choice of metal ion. TALON Superflow is a high-quality option that offers different selectivity for affinity chromatography, typically providing protein with higher purity compared with nickel-based resins.
Successful use in published research
Applications for this his tag purification resin include:
Purification of his-tagged antibody fragments to study Bothrops jararacussu venommyotoxicity
Convenient prepacked formats
TALON Superflow resin is available in the following prepacked formats:
HiTrap TALON crude: HiTrap column format for convenient use with a syringe, a peristaltic pump, or a chromatography system
His SpinTrap TALON: Spin columns for screening of small sample quantities using a benchtop centrifuge
His GraviTrap TALON: gravity flow column for simple purification without the need for equipment
His MultiTrap TALON 96-well plate: for high throughput screening and small scale purification using centrifuge or vacuum equipment