Overview
Biacore Sensor Chip CM5 supports a range of immobilization chemistries to capture nucleic acids, peptides, antibodies, proteins, carbohydrates, or chemical compounds for interaction analysis from research to discovery, development & quality control.
First choice for immobilization via -NH2, -SH, -CHO, -OH, or -COOH groups.
Attach proteins, antibodies, nucleic acids, carbohydrates, and small molecules (<1000 Da).
Compatible with a wide range of applications from basic research to quality control.
Suitable for ligand fishing and fragment-based drug discovery (FBDD).
High immobilization capacity permits a broad range of capture densities.
Obtain real-time data on binding specificity, affinity, kinetics, concentration and more.
Biacore Sensor Chip CM5 Matrix
The CM5 sensor chip cahhies a matrix of carboxymethylated dextran covalently attached to a gold surface. Molecules can be covalently coupled to the sensor surface by exploiting available amine, thiol, aldehyde, or carboxyl functional groups on the ligand. The CM5 dextran matrix extends about 100 nm from the gold surface, and is flexible, allowing relatively free movement of attached ligands.
An exceptionally versatile chip for SPR interaction analysis
Sensor Chip CM5 is the most versatile and widely used dextran-based chip, suitable for most general-purpose surface plasmon resonance (SPR) applications. Carboxymethyl surface chemistry supports the study of many different types of ligands and binding partners–from small organic molecules, such as drug candidates, through to large molecular assemblies and even whole viruses. High binding capacity gives a high response, advantageous for capture assays and for interactions involving small molecules. High surface stability provides accuracy and precision, and allows repeated analysis on the same surface.
Application examples include:
FBDD-based screening for novel drugs against Hepatitis C virus (HCV)
Surface plasmon resonance-based determination of adenovirus concentration on Biacore T200
Sensor chip formats for Biacore systems
Biacore sensor chips are available in two formats:
S series sensor chips are for use with Biacore 1 series, Biacore 8 series, Biacore T200, Biacore S200 and Biacore 4000 SPR systems.
Sensor chips not designated with S series are designed for Biacore X100, and Biacore C SPR systems.